Composite

Part:BBa_K2969050:Design

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-16)


J23119-CI434ts-TEVsite-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 754


Design Notes

None


Source

Zheng, Y., Meng, F., Zhu, Z., Wei, W., Sun, Z., Chen, J., . . . Chen, G.-Q. (2019). A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. Nucleic Acids Research. doi:10.1093/nar/gkz785 In this paper, the author combined the thermosensitive proteolytic with transcriptional regulatory parts to build a tight cold-inducible switch and put it into various applications

References